MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT MGLUR2 RECEPTOR

Product Information

Catalog Number:
DC1189a

Lot Number:
DC1189a-033011

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human GRM2 cDNA with FLAG tag sequence at N- terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM with GlutaMAX (Gibco 10566), 10% FBS (dialyzed), 2 mM sodium pyruvate

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: The neurotransmitter L-glutamate interacts with both ionotropic and metabotropic receptors. The metabotropic glutamate receptors (mGluRs), which are G protein-coupled receptors, have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group II and group III mGluRs are linked to the inhibition of the cyclic AMP cascade, but differ in their agonist selectivity. The mGluR2 is a member of group II metabotropic glutamate receptors.

Application: Functional assays

Figure 1. Dose-dependent inhibition of forskolin-stimulated intracellular cAMP level upon treatment with ligand, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01).

References:

Flor et al. (1995) Molecular cloning, functional expression and pharmacological characterization of the human metabotropic glutamate receptor type 2. Eur J Neurosci 7:622-629.

FOR RESEARCH USE ONLY.
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