MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT LPA5 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human LPA5 cDNA (GenBank accession number NM_020400.5) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: LPA5 (GPR92) represents a putative orphan G protein coupled receptor with unknown functions. ESTs for LPA5 have been isolated from human normal brain, testis, and tonsil B-cell libraries and from a human blood cancer cell library (pre-B cell). LPA5 protein contains 372 amino acids and shared 36 to 40% sequence identity in the transmembrane regions with the G protein-coupled purinergic receptor P2Y5 (P2RY5), GPR23, and GPR17. Recently, functional characteristics of GPR92/LPA5 have been elucidated that demonstrate the receptor’s identity as an LPA receptor.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Lee et al. (2001) Discovery and mapping of ten novel G protein coupled receptor genes. Gene 275:83-91.
White et al. (2000) Autosomal dominant hypophosphataemic rickets is associated with mutations in FGF23. Nat Genet 26:345-348.
Lee et al. (2006) GPR92 as a new G12/13- and Gq-coupled lysophosphatidic acid receptor that increases cAMP, LPA5. J Biol Chem 281:23589-23597.