MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT GPR65 RECEPTOR
1 vial (4 x 106) frozen cells
Expression vector containing full-length human GPR65 cDNA (GenBank Accession Number NM_003608) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Background: GPR65 also known as T cell death-associated gene 8 (TDAG8) is a proton-sensing GPCR and plays a major role in pH homeostasis. This receptor is primarily expressed in lymphoid tissues (spleen, thymus, leukocytes and lymph nodes) and cancer tissues. The major function of this receptor is to reduce immune-mediated inflammation by regulating cytokine production from T cells and macrophages.
Application: Functional assays
Figure 1. Dose-dependent increase of intracellular cAMP level upon treatment with pH, measured with MultiscreenTM TR-FRET cAMP 1.0 No Wash Assay Kit (Multispan MSCM01)
Ludwig, M.-G., Vanek, M., Guerini, D., Gasser, J. A., Jones, C. E., Junker, U., Hofstetter, H., Wolf, R. M., Seuwen, K.Proton-sensing G-protein-coupled receptors. Nature 425: 93-98, 2003.
Saxena, H., Deshpande, D., Tiegs, B., Yan, H., Battafarano, R., Burrows, W., Penn, R. (2012). The GPCR OGR1 (GPR68) mediates diverse signalling and contraction of airway smooth muscle in response to small reductions in extracellular pH. British Journal of Pharmacology, 166(3), 981–990.
Satoshi Ishii, Yasuyuki Kihara and Takao Shimizu (2005) Identification of T Cell Death- associated Gene 8 (TDAG8) as a Novel Acid Sensing G-protein-coupled Receptor. J Biol Chem 280: 9083-9087