MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT LPA3 RECEPTOR

Product Information

Catalog Number:
DC1053-6

Lot Number:
03/07/13

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
RH7777

Transfection:
Expression vector containing full-length human LPAR3 cDNA (GenBank Accession Number NM_012152) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: LPA and the structurally related lysophoslipid mediator sphingosine 1-phosphate (S1P) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1) are S1P receptors; others (e.g., EDG2) are LPA receptors. LPA3 receptor (EDG7) mediates responses preferentially to unsaturated LPA, whereas LPA2 receptor (EDG4) mediates responses to both saturated and unsaturated LPA.

Application: Functional assays

Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).

References:

Bandoh et al. (1999) Molecular cloning and characterization of a novel human G-protein- coupled receptor, EDG7, for lysophosphatidic acid. J. Biol. Chem. 274:27776-27785.

Ye et al. (2005) LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature 435:104-108.

FOR RESEARCH USE ONLY.
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