MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT LPA3 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human LPAR3 cDNA (GenBank Accession Number NM_012152) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: LPA and the structurally related lysophoslipid mediator sphingosine 1-phosphate (S1P) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1) are S1P receptors; others (e.g., EDG2) are LPA receptors. LPA3 receptor (EDG7) mediates responses preferentially to unsaturated LPA, whereas LPA2 receptor (EDG4) mediates responses to both saturated and unsaturated LPA.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Bandoh et al. (1999) Molecular cloning and characterization of a novel human G-protein- coupled receptor, EDG7, for lysophosphatidic acid. J. Biol. Chem. 274:27776-27785.
Ye et al. (2005) LPA3-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature 435:104-108.