MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT OX2 RECEPTOR
1 vial (4 x 106) frozen cells
Full-length Human HCRTR2 cDNA (GenBank Accession Number NM_001526.2) with FLAG-tag sequence at the N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DME/F12, 10% FBS
Background: Orexins, or hypocretins, produced by a small group of neurons in lateral hypothalamus, are involved in many physiological functions such as feeding, energy metabolism, sleep, arousal, reward, substance abuse, stress, as well as sympathetic and cardiovascular functions. OX2 is one of the two receptors for the orexins. The receptor is expressed in the brain regions such as the hypothalamus, hippocampus and thalamus. Recent studies have indicated that orexins may also be useful in the treatment of schizophrenia and other psychiatric disorders.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Deutch and Bubser (2007) The orexins/hypocretins and schizophrenia. Schizophr Bull 33:1277-1283.
Smart et al. (1999) Characterization of recombinant human orexin receptor pharmacology in a Chinese hamster ovary cell-line using FLIPR. Br J Pharmacol 128:1-3.