MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT CCR8 RECEPTOR
1 vial (4 x 106) frozen cells
Expression vector containing full-length human CCR8 cDNA (GenBank Accession Number: NM_005201.2) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1 – 2 days after thawing
Background: CCR8 (C-C chemokine receptor type 8) is a receptor for the chemokines CCL1 (I-309). The receptor regulates monocyte chemotaxis and thymic cell line apoptosis, and is an additional alternative coreceptor with CD4 for HIV-1 infection. CCR8 plays a role on cell types of relevance to respiratory diseases such as asthma, chronic obstructive pulmonary disease (COPD) and chronic bronchitis. The interference of CCR8 from its ligand represents a new approach in allergy therapy.
Figure 1. Receptor expression on cell surface measured by flow cytometry (FACS) using an anti-FLAG antibody. Thin line: parental cells; thick line: receptor-expressing cells.
Simmons et al. (2000) Co-receptor use by HIV and inhibition of HIV infection by chemokine receptor ligands. Immunol Rev 177:112-126.
Garcia et al. (2005) New chemokine targets for asthma therapy. Curr Allergy Asthma Rep 5:155-160