MULTISCREEN™ DIVISION ARRESTED CELL LINE

HUMAN RECOMBINANT CCR7 RECEPTOR

Product Information

Catalog Number:
DC1015

Lot Number:
01/16/13

Quantity:
1 vial (4 x 106) frozen cells

Freeze Medium:
Sigma Freezing Medium (C-6164)

Host cell:
HEK293T

Transfection:
Expression vector containing full-length human CCR7 cDNA (GenBank Accession Number: NM_001838) with FLAG tag sequence at N-terminus

Recommended Storage:
Liquid nitrogen upon receiving

Propagation Medium: DMEM, 10% FBS

Stability:
Stable for 1-2 days after thawing

Data Sheet

Background: CCR7 (C-C chemokine receptor type 7 is a receptor for the chemokine MIP- 3β. CCR7 has been identified as a key regulator of homeostatic B and T cell trafficking to secondary lymphoid organs. CCR7 is also an essential mediator for entry of both dermal and epidermal dendritic cells into the lymphatic vessels within the dermis. CCR7 plays an important role in tumor cell migration and lymph node metastasis. Expression of CCR7 is related to the development of lymph node metastasis in nonsmall cell lung cancers.

Application: Functional assays

Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Cells were transiently transfected with Gαqi5 before division arrest.

References:

Takanami (2003) Overexpression of CCR7 mRNA in nonsmall cell lung cancer: correlation with lymph node metastasis. Int J Cancer 105:186-189.

Ohl et al. (2004) CCR7 governs skin dendritic cell migration under inflammatory and steady- state conditions. Immunity 21:279-288.

FOR RESEARCH USE ONLY.
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