MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT CCR6 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR6 DNA (GenBank Accession Number NM_004367) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: CCR6 is a receptor for macrophage inflammatory protein (MIP)-3α (CCL20). It is expressed in immature dendritic cells as well as in B lymphocytes and memory T cells. In gene-targeted mice lacking CCR6, myeloid dendritic cells are either absent or underrepresented in the subepithelial dome compared with wild-type mice. CCR6 and its ligands contribute to the normal homeostatic regulation of extrathymic T cell development in the intestine.
Application: Functional assays
Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01).
Baba et al. (1997) Identification of CCR6, the specific receptor for a novel lymphocytedirected CC chemokine LARC. J Biol Chem 272:14893-14898.
Cook et al. (2000) CCR6 mediates dendritic cell localization, lymphocyte homeostasis, and immune responses in mucosal tissue. Immunity 12:495-503.
Lugering et al. (2003) Lymphoid precursors in intestinal cryptopatches express CCR6 and undergo dysregulated development in the absence of CCR6. J Immunol 171:2208-2215.