MULTISCREEN™ DIVISION ARRESTED CELL LINE
HUMAN RECOMBINANT CCR2 RECEPTOR
1 vial (4 x 106) frozen cells
Sigma Freezing Medium (C-6164)
Expression vector containing full-length human CCR2 cDNA (GenBank accession number NM_000647.3) with FLAG tag sequence at N-terminus
Liquid nitrogen upon receiving
Propagation Medium: DMEM, 10% FBS
Stable for 1-2 days after thawing
Background: CCR2 (C-C chemokine receptor type 2 or monocyte chemoattractant protein 1 receptor) is a receptor for the MCP-1, MCP-3 and MCP-4 chemokines. It is also an alternative coreceptor with CD4 for HIV-1 infection. CCR2 has been widely considered as a potential therapeutic target for autoimmune disease and inflammatory disorders of the lung, particularly rheumatoid arthritis, and various CCR2 blocking agents have been developed, some of which might have therapeutic values.
Application: Functional assays
Figure 1. Dose-dependent stimulation of calcium flux upon treatment with ligand, measured with MultiscreenTM Calcium 1.0 No Wash Assay Kit (Multispan MSCA01). Cells were transiently transfected with Gαqi5 before division arrest.
Quinones et al. (2005) The complex role of the chemokine receptor CCR2 in collagen-induced arthritis: implications for therapeutic targeting of CCR2 in rheumatoid arthritis. J Mol Med 83:672- 681.
Rose et al. (2003) Significant involvement of CCL2 (MCP-1) in inflammatory disorders of the lung. Microcirculation 10:273-288.