GPCR Expression Technology
Multispan uses a proprietary technology to enable expression of GPCR proteins in mammalian cells at over a million molecule per cell surface. High expressing cells (HExTM
) can be developed into single cell clones and be used as unique source of high quality GPCR proteins for variety of applications, including:
- Antibody generation using antigens with natural epitopes at high expression level
- Cell-based or membrane receptor binding assays to achieve high signal-to-noise ratio
- Cell-based functional assays for hard-to-express GPCRs from all 3 structurally distinctive GPCR families, including Free Fatty Acid, Lysophospholipid, Prostanoid, Neuropeptide, Purinergic and Metabotropic Glutamate receptors.
- Structural studies as high quality source of protein for crystallization for antibody generation and membranes for binding assays for high throughput screening.